Additionally, a comparison of a diet with dietary protein at recommended maintenance level (CP: 21.5 g/100 g DM; CF: 31.0 g/100 g; and carbohydrates: 49.0 g/100 g DM) and a HP diet (CP: 33.8 g/100 g DM; HF: 29.4 g/100 g DM; and carbohydrates: 38.6 g/ 100 g DM) did not cause alterations in basal concentrations of glucagon, leptin, insulin and insulin‐like growth factor 1 (IGF‐1) (Kawauchi et al., 2017). This evidence concerns the gene INS and hydrops fetalis.