Recently, a 3D human AD triculture system containing neurons, astrocytes, and microglia in a 3D microfluidic platform was developed by Park et al. [78] This model possesses most of the key AD features such as Aβ aggregation, p-tau accumulation, and neuroinflammatory activity such as microglia recruitment and neurotoxic activity (including axonal cleavage). Here, MAPT is linked to Alzheimer disease.