Additionally, the insulin signaling pathway and amoebiasis were enriched with DEGs in the egg stage (Figure 5A), and many DEGs were enriched in metabolic pathways, amoebiasis, vibrio cholera infection, vascular smooth muscle contraction, platelet activation, oxytocin signaling pathway, amino sugar and nucleotide sugar metabolism, and glycolysis/gluconeogenesis in the 3rd-instar nymph stage (Figure 5B). Here, INS is linked to amebiasis.