To begin to investigate whether pre-existing anti-PEG antibodies can affect the therapeutic efficacy of PEG-EPO in patients, the concentration of anti-PEG IgM and IgG antibodies were measured in serum samples from 39 peritoneal dialysis patients suffering from anemia who did not respond well to PEG-EPO and 16 control patients who did respond to PEG-EPO. Here, CD40LG is linked to anemia.