To confirm that ACC tumors used the TSS2 promoter, we mapped the 5′-ends of transcripts in ACC tumor T73 using RNA-Ligase Mediated-Rapid Amplification of cDNA Ends (5′RLM-RACE), followed by conventional ‘Sanger’ sequencing (Figure 1B). Here, RLIG1 is linked to adrenal cortex carcinoma.