TERF1 and seminoma: Both the semi-quantitative RT-PCR using the primer pair which detects all TERF1 variants (Figure 11A) and the real-time RT-qPCR using the isoform-specific primers (Figure 11B) show a clear downregulation of TERF1-tsi in seminoma tumors in comparison to the matched normal tissue sample from the same individual while TERF1 or PIN2 mRNA levels are not significantly different.