Cgas-/- iMOs were more susceptible to infection (Fig 4O) and did not upregulate IFN-I (Ifna4 (Fig 4P), Ifna-non4 (S3M Fig) and Ifnb1 (Fig 1N) or the ISGs (Ifit3 (Fig 4Q), Irf7 (S3O Fig), Isg15 (S3P Fig) and Mx1 (S3Q Fig) but did not differ from B6 iMOs in the frequencies that were CXCL9+ or TNF-α+ (S3R and S3S Fig). This evidence concerns the gene ISG15 and infection.