To evaluate the role of the negative correlation between the miR-548 cluster and HMGB1 in preterm birth with acute chorioamnionitis, hAECs were treated with lipopolysaccharide (LPS) (10 or 100 ng/mL) for 24 h to induce inflammation followed by measurement of the expression levels of the miR-548 cluster and HMGB1. This evidence concerns the gene HMGB1 and chorioamnionitis.