Multivariate analysis adjusted to resection margins and lymph node metastasis confirmed that the negative SLC22A3 IHC brush border staining presents high risk factor for disease progression (PFS) (P = 0.049, hazard ratio = 2.3, 95% confidence interval = 1.0–5.1), but not for death (OS). The gene discussed is SLC22A3; the disease is metastatic malignant neoplasm in the lymph nodes.