In accordance to the demonstrated strong expression levels and nuclear YAP1/TAZ immunoreactivity in primary tumor tissue specimens, fractionation experiments demonstrated that YAP1 and TAZ were predominantly localized in the nucleus of MLS, SySa and MPNST cells (Fig. 2G), whereas YAP1 and TAZ were predominantly localized in the cytoplasm of TC-32 EwS cells (comparable to the low YAP1/TAZ nuclear expression detected by IHC), providing evidence that YAP1/TAZ signaling might represent a unifying feature in MLS, SySa and MPNST. Here, WWTR1 is linked to malignant peripheral nerve sheath tumor.