The result showed that the tumor cell migration of both shRNA mutants in NES‐p27 and 143B was significantly reduced when compared with the scramble controls, while the migration of U2OS was significantly reduced in the shRNA#1 mutant only, confirming the tumor cell migration was dependent on PAK1 expression and the migration results of the PAK inhibitor studies (Fig. 3B,C). This evidence concerns the gene PAK1 and neoplasm.