Low levels of hCLCA1 expression in the colonic epithelial tissues correlated with a less differentiated tumor histological grade, an advanced tumor stage, metastases in regional lymph nodes, and high Dukes stage that has been used to grade colon cancer based on cancer cell locations from the mucosa, muscle layer, to lymph node, and distant metastasis. Here, CLCA1 is linked to colonic neoplasm.