In general, our study has included several important substances: NVP-BEZ235 induce autophagy through AMPK/ULK1 pathway; Blocking autophagy by knocking down AMPK or ULK1 promote NVP-BEZ235 induced apoptosis; The combinational therapy of NVP-BEZ235 and CQ shows synergistic antitumor effects in colon cancer in vitro and in vivo. Here, ULK1 is linked to malignant colon neoplasm.