We investigated the effect of exposure to OGD/R, hypothermia, and pyrexia on the inducible NO synthase (iNOS) expression in the HL-1 cardiomyocytes (see Figure 2) and observed a significant increase in iNOS expression relative to normoxia control after exposure to OGD that was not attenuated by the brief period of hypothermia (6 h), but no significant increases were observed in the reperfusion phase (8–27 h). This evidence concerns the gene NOS2 and Fever.