In both the inner and outer areas of the dentate gyrus, and across the whole hippocampus and within dorsal and ventral subregions, HF diet increased Iba1+ area in males only [p < 0.05 for all except outer ventral CON male vs HF male (p = 0.1137); mean differences ranging from 76.09% to 120.00%; Fig. 6A,B). Here, AIF1 is linked to hydrops fetalis.