The NF-kB immunostaining was significantly higher in tumour and lymph node metastasis than in MSR squamous epithelium (p<0.001), and the TNF-α immunostaining was significantly higher in tumour than in lymph node metastasis and MSR squamous epithelium (p<0.001). The gene discussed is TNF; the disease is metastatic malignant neoplasm in the lymph nodes.