And as predicted based on previous literature, gdf6a(lf) mutants and BMPi-treated embryos showed a decrease in expression of the chondrocyte marker, col2a1a. We observed no changes in foxd3 expression, but observed a slight downregulation of sox10 (Figure 5—figure supplement 1A), previously shown to be downregulated upon GDF6 knockdown in melanoma cells (Venkatesan et al., 2018). Here, FOXD3 is linked to melanoma.