Then, rescue experiments were performed with WT CPT1C and two mutated proteins: CPT1CR37C, the missense mutation associated with HSP (Rinaldi et al., 2015); and CPT1CM589S, a mutation that maintains CPT1 functionality but prevents the binding of malonyl-CoA (Morillas et al., 2003; Rodríguez-Rodríguez et al., 2019). Here, CPT1C is linked to hereditary spastic paraplegia.