To investigate further the contribution of DNA binding to MeCP2 dynamic behavior, we targeted the MBD domain with two common Rett syndrome mutations, known to disrupt MeCP2 DNA binding to different degrees (Cuddapah et al., 2014): R106W, which completely abolishes DNA binding and R133C which retains significant DNA binding capacity (Nikitina et al., 2007). This evidence concerns the gene MECP2 and atypical Rett syndrome.