To clarify the role of Nedd4 and Nedd4l in intestinal tumorigenesis, we further crossed the mutant mice to Apcmin animals, a mouse model of colon cancer (Su et al, 1992), to obtain Apcmin Nedd4 cKO, Apcmin Nedd4l cKO, and the compound Apcmin DKO mice. Here, NEDD4 is linked to malignant colon neoplasm.