In this study, we evaluated IgG seroreactivity against the gene products BBA69 and BBA73 together with antigens OspC, DbpA, FlaB, and VlsE in Stage 1 and Stage 2 early Lyme disease patient serum samples, and combined IgM and IgG responses in a multi-antigen approach for sensitivity and specificity determination. The gene discussed is YBX3; the disease is Lyme disease.