Lastly, qRT-PCR (Fig. 4d) and immunohistochemistry (Fig. 4e) analysis revealed that the up-regulation of PRC1 in the livers of LF mice injected with Ad-shNC were down-regulated by Ad-shPRC1 injection, suggesting that knockdown of PRC1 attenuated LF progression. This evidence concerns the gene PRC1 and Lassa fever.