Dependency score analysis by DepMap showed that ACTG1 and RHOQ were less essential across pan-cancer cells including COAD cell lines (Supplementary Figures 2A, B), and CCT6A, UTP18, YRDC, RRP12, RFT1, NLE1, as well as DDOST were essential across pan-cancer cells including most of COAD cell lines (Figures 9A–G). This evidence concerns the gene NLE1 and cancer.