Lastly, pan-cancer analysis of dependency score (Meyers et al., 2017; Tsherniak et al., 2017) showed that CCT6A, UTP18, YRDC, RRP12, RFT1, NLE1, as well as DDOST were essential across pan-cancer including COAD cells, while ACTG1 or RHOQ were less essential in cancer cells, which indicated that the essential genes would be developed into anticancer drugs as potential targets. This evidence concerns the gene NLE1 and cancer.