In addition, prognostic significance analysis demonstrated that high expressions of CCT6A, RHOQ, and RRP12 were relative to lower survival rates (OS or RFS) in COAD patients or subtypes, which could be interpreted as promoting growth or viability of COAD cells because deletion of each of the above genes inhibited the growth of at least three COAD cell lines according to our fitness gene mining results. This evidence concerns the gene RHOQ and colon adenocarcinoma.