Next, we showed that the expression of HIF1A-AS2 was upregulated in osteosarcoma cell lines (U2OS, SoSP-M, SaOS-2, MG-63) compared to the expression in the osteoblast cell line (hFOB) (Figure 2A, p<0.001). The gene discussed is HIF1A; the disease is osteosarcoma.