The expression of HIF1A-AS2 was significantly higher in the osteosarcoma samples compared to the expression levels in the paired normal controls (Figure 1A, mean expression in the osteosarcoma samples versus the normal tissue = 2.790 ± 0.2451 vs. 1.321 ± 0.1329, respectively; p<0.001). This evidence concerns the gene HIF1A and osteosarcoma.