POLE and endometrial cancer: To further investigate the role of POLE mutations in endometrial cancer, we used transcriptome sequencing data from 526 patients to analyze the global gene expression profile and the differential expression between the two groups in 80 POLE mutant patients and 446 patients with wild type POLE. A total of 1587 differentially expressed genes (DEGs) were obtained according to the cut-off criteria (P < 0.05 and FC > 1.5) including 725 upregulated genes and 862 downregulated genes (Figs. 3a).