Both NO itself and ONOO• can regulate a variety of molecular expression and function.17, 21, 22, 23 Importantly, it was found that sodium nitroprusside (SNP), a type of NO donors, causes anxiogenic effect at high doses but exerts anxiolytic property at low doses, implicating ONOO• in the induction of anxiety behaviors.24, 25, 26 Here, using genetic and pharmacological methods, we investigated the link among glucocorticoids, nNOS, and 5‐HT1A receptor. Here, HTR1A is linked to Anxiety.