NOS1 and Anxiety: Systematic inhibition of nNOS activity prevented anxiety behavior change (Figure 4 A‐B, one‐way ANOVA, central distance: F2,33 = 7.45, P < .01; central time: F2,33 = 29.00, P < .001, n = 10‐12) after CUMS exposure without locomotor activity alteration as measured in the OPT (Figure 4C).