In this study, we generated the RAI16 knockout (RAI16−/−) mouse model by CRISPR/Cas9 strategy, in order to evaluate the function of RAI16 by comparing RAI16−/− mice with wild type mice in dextran sulfate sodium (DSS) induced colitis and azoxymethane (AOM)-DSS induced CAC mouse models. The gene discussed is FHIP2B; the disease is colitis.