A primer library was generated containing (I) the 8 previously-linked EDMD gene ORFs plus the whole genes for LMNA and EMD (that together account for ~40% of linked alleles), (II) 25 genes from similar muscular dystrophies, (III) the 252 exome sequencing candidates, and (IV) 16 functional candidates, mostly muscle-specific nuclear envelope proteins (Fig. 2a; Supplemental Table S4). This evidence concerns the gene SUN2 and Emery-Dreifuss muscular dystrophy.