In the present study, C57Bl/6 mice were subcutaneously injected with 1 × 106 B16 cells and the application of Hsp70-containing gel or control gel (vehicle) was performed onto preliminarily shaven areas of skin surrounding a 7-day-old B16 tumor; the application was repeated every 3 days until the end of experiments. Here, HSPA1A is linked to neoplasm.