To approve the data obtained in vitro, we examined the amount of TAMs inhabiting B16 melanoma tumors that were treated with exogenous Hsp70; in this experiment, we showed that chaperone delivery caused significant reduction of pro-cancer macrophages and resorption of multicellular colonies formed by arginase-1-positive cells in the absence of Hsp70 (Figure 7). This evidence concerns the gene ARG1 and melanoma.