To this end, colonospheres (Figure 7A) were co-cultured with primary IL-2 and IL-15 pre-activated and expanded CD45-APC stained NK cells at 1:5 ratio and the tumor spheroid integrity, NK infiltration and cytotoxicity was monitored by light and fluorescence microscopy 48 h latter (Figure 7). The gene discussed is PTPRC; the disease is neoplasm.