To achieve mitochondria-targeted EISA for cancer therapy, the self-assembling peptide NBD-FFYpK-TPP was synthesized bearing 4-nitro-2,1,3-benzoxadiazole (NBD) as a fluorophore, FFYK (phe-phe-tyr-lys)as a self-assembling peptide unit, tyrosine phosphate (Yp) as an ALP (alkaline phosphatase) enzymatic substrate, and TPP as a mitochondria-targeting unit. The gene discussed is TNS3; the disease is cancer.