SIRT1 and colorectal cancer: Indeed, overexpression of the lncRNA H19, a sponge of miRNA, or its accumulation following 5-FU treatment in colorectal cancer cells (HCT8 and HCT116) inhibits miR-194-5p, a negative regulator of SIRT1, and thus increases SIRT1 expression and autophagy (increase of autophagosomes, LC3B-II, and decrease of SQSTM1/P62) [118].