To study whether inactivation of SESN1/2 affects phosphorylation of AKT in human lung cancers, A549 cell lines inactivated for SESN1, SESN2, or both genes using CRISPR/Cas9 constructs were prepared and immunoblots confirmed that the respective genes were inactivated (Figure 4C). The gene discussed is SESN1; the disease is lung cancer.