To study whether inactivation of SESN1/2 affects phosphorylation of AKT in human lung cancers, A549 cell lines inactivated for SESN1, SESN2, or both genes using CRISPR/Cas9 constructs were prepared and immunoblots confirmed that the respective genes were inactivated (Figure 4C). This evidence concerns the gene SESN1 and lung carcinoma.