The association between the coexistence of HBsAg and anti-HBs and HBsAg seroclearance (HR 1.43, 95% CI 1.01–2.03, P = 0.046) remained significant even after adjustment for covariates including age, sex, ALT level, presence of cirrhosis, significant fibrosis evaluated by FIB-4, history of antiviral medication, and qHBsAg level (Table 3 and Fig. 2b). This evidence concerns the gene GPT and Cirrhosis.