NEDD4 and ductal breast carcinoma in situ: All subtypes of BC in different stages of BC development displayed increased NEDD4 expression compared to ANT (Fig. 3a, DCIS vs. ANT, χ2 = 19.166, P < 0.001; IDC vs. ANT, χ2 = 62.183, P < 0.001; BCLNM vs. ANT, χ2 = 98.271, P < 0.001; IDC vs. DCIS, χ2 = 0.647, P = 0.421; BCLNM vs. DCIS, χ2 = 6.835, P = 0.009; BCLNM vs. IDC, χ2 = 9.530, P = 0.002).