In total, 79% (22/28) of the proteins encoded by previous reported hypospadias risk associated genes [11] directly or indirectly interacted with SP1 and SP7, whereas six proteins (DGKK, SRD5A2, HOXA4, HOXB6, MAMLD1, BNC2) were not found to be interacted with SP1 and SP7. The gene discussed is SP1; the disease is hypospadias.