Based on the expression levels of these nine significant markers (p53, p62, HCCR, C‐myc, MDM2, miR‐21, miR‐223, miR‐375, and miR‐100), we found that a panel of six plasma markers (HCCR, C‐myc, MDM2, miR‐21, miR‐223, and miR‐375) was able to effectively discriminate the ESCC patients from control individuals. Here, MYC is linked to esophageal squamous cell carcinoma.