Our previous research showed that the five miRNAs (miRNA‐21, miRNA‐375, miRNA‐223, miRNA‐100, and miRNA‐25) had better diagnostic ability for patients with esophageal cancer.23 In the present study, we evaluated the diagnostic value of seven TAAbs (p53, p62, HCCR, C‐myc, MDM2, hnRNPA2B1, and NICD), and further explored the potential application of combining TAAbs with miRNAs in detection of ESCC. The gene discussed is LETMD1; the disease is esophageal squamous cell carcinoma.