To determine whether they were required for Dlg5’s function in BC migration, we overexpressed aPKC or Arm to perform rescue of the BC migration delay that was resulted from RNAi knockdown of dlg5. As mentioned above, expressing dlg5. RNAi in BC clusters caused ~40% migration delay (Fig 1F, 1G and 1I). The gene discussed is DLG5; the disease is breast cancer.