TSC2 and lymphangioleiomyomatosis: In addition to the 86 significant genetic variants identified in TSC1 and TSC2, 61 variants in 31 non-TSC1/2 genes related to tumorigenesis were found in the 37 LAM patients, including 1 frame-shift deletion (ID130), 1 in-frame insertion (ID054), 3 nonsense mutations (ID086, ID150, and ID162), 2 splicing mutations (ID015 and ID135), and 54 missense mutations (S6 Table, Fig 5).