As expected, the inhibition of AKT/eNOS signalling pathway increased the expression of ANP, BNP and MYH7 in cardiomyocytes treated with S1pr1‐overexpresssing EC medium (Figure 4B‐D), suggesting that EC‐S1pr1 inhibited the pathophysiological process of cardiac hypertrophy via AKT/eNOS signalling pathway. This evidence concerns the gene NOS3 and cardiac hypertrophy.