APOE and early-onset autosomal dominant Alzheimer disease: Western blot analysis of homogenate of temporal cortex from post-mortem brain (Fig. 2A, un-cropped western blots are shown in Supplementary Figs 1 and 2) shows an increase in the amount of clusterin in Alzheimer's disease compared with control, with no effect of APOE genotype on this increase (two-way ANOVA effect of disease F (1, 30) = 16.96, P = 0.0003, effect of APOE genotype F (1, 30) < 0.0001, P = 0.998; Tukey’s post hoc between control APOE3 and Alzheimer's disease APOE3 P = 0.0241, Tukey’s post hoc between Control APOE4 and Alzheimer's disease APOE4 P = 0.0421, Fig. 2C).