NANOG and neoplasm: Moreover, immune-histochemistry staining and semiquantification analysis of the IHC data showed that subcutaneous tumours arising from depletion of miR-4319 in Hep3B cells increased the expression of EMT (E-cadherin and vimentin) and CSC (Nanog, Sox2, CD44 and EPCAM) markers in comparison to that in the control group (Figure 4D).