TNNT3 and myotonic dystrophy type 1: RT-qPCR analysis demonstrated the misregulation of Ldb3, Serca1, m-TTN, Tmem63b, Sorbs1 and Spag9 mRNA splicing, while the splicing efficiencies of Clcn1, Ryr1, Ryr2 and Tnnt3 mRNAs were normal (Supplementary information, Fig. S8; data not shown), reflecting the heterogeneity of mis-splicing in patients.62,63 A few mis-spliced genes observed in human patients appeared to be normal in our model, suggesting that other splicing factors such as MBNL264 and CUGBP159 might be involved in DM1.