Meanwhile, we could not exclude the possibility that the potential dosage changes of genes in H19/Igf2 and Dlk1/Dio3 imprinted clusters induced by H19-DMR and IG-DMR deletions in DKO-AG-haESCs may partially account for the complex symptoms of the models.75 Moreover, while our models can recapitulate most symptoms of DM1 patients, they do not exhibit a few typical DM1 features, such as abnormal Clcn1 splicing, implying that other DM1-related genes including MBNL276 and CUGBP159 might be involved in the disease. The gene discussed is CLCN1; the disease is myotonic dystrophy type 1.