In six HCC and GI cancer cell lines, we observed dramatically markedly expression of TP73, cytosine residues of CpG dinucleotides in the TP73 promoter region (−1479 to −1226), which were almost completely methylated, whereas those cytosine residues in normal cell lines (HepCY, HepCO and HS27), which lost TP73 expression, were entirely methylation-free (Fig. 3C). This evidence concerns the gene TP73 and hepatocellular carcinoma.