We further analyzed related genes (ALB, AFP, CTCF, MET, TP53 and PIK3CA) in human normal liver stem, hepatocellular cancer (HCC) cell lines, gastrointestinal cancer (GI) cell lines and normal skin fibroblasts cell line by using RT-PCR (Fig. 2BII). This evidence concerns the gene PIK3CA and hepatocellular carcinoma.