Then, the expression of ZFAS1 in the tumour xenografts was measured using qRT‐PCR, and the results are shown in Figure 3D. ZFAS1 was obviously down‐regulated in the tumour xenografts formed by shZFAS1‐transfected HONE1 cells but up‐regulated in the tumour xenografts formed by pZFAS1‐transfected HONE1 cells (Figure 3D, P < .01). This evidence concerns the gene ZFAS1 and neoplasm.