Iron homeostasis has been previously associated with adiposity, perhaps mediated by obesity induced inflammation.[38] In one study of mice fed high fat diets, TFR2 was more highly expressed in adipose tissue compared to mice fed a usual diet.[35] However, the direction of our findings differ from this report, as in our study, increased TFR2 methylation was positively associated with leptin levels. This evidence concerns the gene TFR2 and obesity disorder.