Figure 5C shows that the top 10 regulons are consistently HCC-responsive. Regulons consist of both MR-induced and MR-repressed genes, and their relative activity influences the phenotype. To evaluate regulon activity, two-tailed GSEA was performed to calculate differential enrichment scores (dES) based on positively and negatively regulated regulon subsets in HCC samples. Detailed target information for each MR is provided in Supplementary Table 6. This evidence concerns the gene NR3C2 and hepatocellular carcinoma.