In this study, the relative expression level of PLP2 in the inhibitor group was significantly lower than that in the mimics group and the NC group, and the level in the mimics group was significantly higher than that in the NC group, which indicates that miR-664-3p may play a role in the proliferation and apoptosis of osteosarcoma cells by regulating the expression of PLP2. This evidence concerns the gene PLP2 and osteosarcoma.