The median of CDH1 relative quantification (RQ) was 0.7 (0.2–9.5), 0.9 (0.2–5.6), 0.5 (0.02–1.7), and 3.45 (0.6–2.4) in normal, PIA, PC and metastases samples, respectively. The gene discussed is CDH1; the disease is pachyonychia congenita.