Importantly, our data highlight that cultivation of NK cells from CD3/CD19-depleted primary material in NK MACS® medium compared to X-VIVOTM10 medium resulted in significantly higher expansion rates up to 26.4-fold compared to 13.2-fold following IL-15low stimulation, which was combined with higher purity and viability, but slightly lower cytotoxicity against NB cell lines. Here, CD19 is linked to neuroblastoma.